Affiliation:
1. Department of Chemistry The University of Hong Kong Pokfulam Road Hong Kong China
2. State Key Laboratory of Synthetic Chemistry The University of Hong Kong Pokfulam Road Hong Kong China
3. Department of Chemistry and Key Laboratory for Preparation Application of Ordered Structural Materials of Guangdong Province Shantou University Guangdong 515063 China
Abstract
AbstractDevelopment of a fluorescent probe for activity‐based sensing of activity of alcohol dehydrogenase, a key enzyme in ethanol biooxidation, is reported. A caged coumarin reporter is released upon the selective oxidation by the enzyme with a strong, >60‐fold emission enhancement. The probe has a low cytotoxicity and has been applied in visualising alcohol dehydrogenase activity in HepG2, A549 and HEK293T cells, demonstrating its potential as a convenient, easy‐to‐use bioanalytical tools in unveiling the roles of the enzyme in alcohol metabolism.
Funder
CAS-Croucher Funding Scheme for Joint Laboratories
University of Hong Kong