Functional characterization of a hypothetical protein (<scp>TTHA1873</scp>) from <i>Thermus thermophilus</i>-Reference-Cited by-同舟云学术

Functional characterization of a hypothetical protein (TTHA1873) from Thermus thermophilus

Published:2023-05-31 Issue:10 Volume:91 Page:1427-1436
ISSN:0887-3585
Container-title:Proteins: Structure, Function, and Bioinformatics
language:en
Short-container-title:Proteins

Author:

Iyyappan Yuvaraj¹²,Dhayabaran Vaigundan³,Elayappan Mohanapriya¹,Chaudhary Santosh Kumar¹⁴,Palaniappan Chandrasekaran¹⁵,Kanagaraj Sekar¹

Affiliation:

1. Department of Computational and Data Sciences Indian Institute of Science Bangalore India

2. National Institute for Plant Biotechnology New Delhi India

3. Genomics and Central Research Laboratory, Department of Cell Biology and Molecular Genetics Sri Devaraj Urs Academy of Higher Education and Research Kolar India

4. Chemical Biology and Therapeutics Sciences Broad Institute of MIT and Harvard Cambridge Massachusetts USA

5. Molecular Biophysics Unit, Indian Institute of Science Bangalore India

Abstract

AbstractThermus thermophilus is an extremely thermophilic organism that thrives at a temperature of 65°C. T. thermophilus genome has ~2218 genes, out of which 66% (1482 genes) have been annotated, and the remaining 34% (736 genes) are assigned as hypothetical proteins. In this work, biochemical and biophysical experiments were performed to characterize the hypothetical protein TTHA1873 from T. thermophilus. The hypothetical protein TTHA1873 acts as a nuclease, which indiscreetly cuts methylated and non‐methylated DNA in divalent metal ions and relaxes the plasmid DNA in the presence of ATP. The chelation of metal ions with EDTA inhibits its activity. These results suggest that the hypothetical protein TTHA1873 would be a CRISPR‐associated protein with non‐specific DNase activity and ATP‐dependent DNA‐relaxing activity.

Publisher

Wiley

Subject

Molecular Biology,Biochemistry,Structural Biology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/prot.26530

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