Vincristine exposure impairs mouse oocyte quality by inducing spindle defects and early apoptosis

Author:

Chang Haoya1ORCID,Cheng Siyu1,Xing Guoqiang2,Huang Chenyang3,Zhang Chunhui1,Qian Weiping1,Li Jian1

Affiliation:

1. Department of Reproductive Medicine Peking University Shenzhen Hospital Shenzhen China

2. Department of General Surgery Tianjin Fifth Central Hospital (Peking University Binhai Hospital) Tianjin China

3. Department of Human Cell Biology and Genetics, School of Medicine Southern University of Science and Technology Shenzhen China

Abstract

AbstractVincristine (VCR) is a microtubule‐destabilizing chemotherapeutic agent commonly administered for the treatment of cancers in patients, which can induce severe side effects including neurotoxicity. In context of the effects on female fertility, ovarian toxicity has been found in patients and mice model after VCR exposure. However, the influence of VCR exposure on oocyte quality has not been elucidated. We established VCR exposure in vitro and in vivo model. The results indicated in vitro VCR exposure contributed to failure of oocyte maturation through inducing defects in spindle assembly, activation of SAC, oxidative stress, mitochondrial dysfunction, and early apoptosis, which were confirmed by using in vivo exposure model. Moreover, in vivo VCR exposure caused aneuploidy, reduced oocyte‐sperm binding ability, and the number of cortical granules in mouse oocyte cortex. Taken together, this study demonstrated that VCR could cause meiotic arrest and poor quality of mouse oocyte.

Funder

National Natural Science Foundation of China

Basic and Applied Basic Research Foundation of Guangdong Province

Publisher

Wiley

Subject

Cell Biology,Clinical Biochemistry,Genetics,Molecular Biology,Biochemistry

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