Affiliation:
1. Department of Biophysics Department of Neurology of the Fourth Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310058 China
2. State Key Laboratory of Modern Optical Instrumentations Centre for Optical and Electromagnetic Research College of Optical Science and Engineering International Research Center for Advanced Photonics Zhejiang University Hangzhou 310058 China
3. Department of Toxicology Department of Medical Oncology of the Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310058 China
4. MOE Frontier Science Center for Brain Research and Brain‐Machine Integration Zhejiang University School of Medicine Hangzhou 310058 China
Abstract
AbstractNear‐infrared (NIR) photothermal manipulation has emerged as a promising and noninvasive technology for neuroscience research and disease therapy for its deep tissue penetration. NIR stimulated techniques have been used to modulate neural activity. However, due to the lack of suitable in vivo control systems, most studies are limited to the cellular level. Here, a NIR photothermal technique is developed to modulate cellular excitability and animal behaviors in Caenorhabditis elegans in vivo via the thermosensitive transient receptor potential vanilloid 1 (TRPV1) channel with an FDA‐approved photothermal agent indocyanine green (ICG). Upon NIR stimuli, exogenous expression of TRPV1 in AFD sensory neurons causes Ca2+ influx, leading to increased neural excitability and reversal behaviors, in the presence of ICG. The GABAergic D‐class motor neurons can also be activated by NIR irradiation, resulting in slower thrashing behaviors. Moreover, the photothermal manipulation is successfully applied in different types of muscle cells (striated muscles and nonstriated muscles), enhancing muscular excitability, causing muscle contractions and behavior changes in vivo. Altogether, this study demonstrates a noninvasive method to precisely regulate the excitability of different types of cells and related behaviors in vivo by NIR photothermal manipulation, which may be applied in mammals and clinical therapy.
Funder
National Natural Science Foundation of China
Subject
General Materials Science,General Chemistry