Live Mapping of the Brain Extracellular Matrix and Remodeling in Neurological Disorders

Author:

Ge Xiaoqian1ORCID,Xu Xueqi1,Cai Qi1ORCID,Xiong Hejian1ORCID,Xie Chen1ORCID,Hong Yi2,Gao Xiaofei3,Yao Yao4,Bachoo Robert356,Qin Zhenpeng1789ORCID

Affiliation:

1. Department of Mechanical Engineering The University of Texas at Dallas Richardson TX 75080 USA

2. Department of Bioengineering University of Texas at Arlington Arlington TX 76010 USA

3. Department of Internal Medicine University of Texas Southwestern Medical Center Dallas TX 75390 USA

4. Department of Molecular Pharmacology and Physiology University of South Florida Tampa FL 33612 USA

5. Department of Neurology and Neurotherapeutics University of Texas Southwestern Medical Center Dallas TX 75390 USA

6. Harold C. Simmons Comprehensive Cancer Center University of Texas Southwestern Medical Center Dallas TX 75390 USA

7. Department of Bioengineering The University of Texas at Dallas Richardson TX 75080 USA

8. Center for Advanced Pain Studies The University of Texas at Dallas Richardson TX 75080 USA

9. Department of Biomedical Engineering University of Texas Southwestern Medical Center Dallas TX 75390 USA

Abstract

AbstractLive imaging of the brain extracellular matrix (ECM) provides vital insights into changes that occur in neurological disorders. Current techniques such as second or third‐harmonic generation offer limited contrast for live imaging of the brain ECM. Here, a new method, pan‐ECM via chemical labeling of extracellular proteins, is introduced for live brain ECM imaging. pan‐ECM labels all major ECM components in live tissue including the interstitial matrix, basement membrane, and perineuronal nets. pan‐ECM enables in vivo observation of the ECM heterogeneity between the glioma core and margin, as well as the assessment of ECM deterioration under stroke condition, without ECM shrinkage from tissue fixation. These findings indicate that the pan‐ECM approach is a novel way to image the entire brain ECM in live brain tissue with optical resolution. pan‐ECM has the potential to advance the understanding of ECM in brain function and neurological diseases.

Funder

National Science Foundation

Foundation for the National Institutes of Health

Publisher

Wiley

Subject

General Materials Science,General Chemistry

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3