Affiliation:
1. Center for Mitochondrial Genetics and Health Greater Bay Area Institute of Precision Medicine (Guangzhou) Fudan University Guangzhou 511458 China
2. State Key Laboratory of Genetic Engineering School of Life Sciences Human Phenome Institute Fudan University Shanghai 200438 China
3. Zhejiang Lab Hangzhou 310000 China
Abstract
AbstractA universal platform is developed for dropletizing single cell plate‐based multiomic assays, consisting of three main pillars: a miniaturized open Heterogeneous Hydrogel reactor (abbreviated HetHydrogel) for multi‐step biochemistry, its tunable permeability that allows Tn5 tagmentation, and single cell droplet barcoding. Through optimizing the HetHydrogel manufacturing procedure, the chemical composition, and cell permeation conditions, simultaneous high‐throughput mitochondrial DNA genotyping and chromatin profiling at the single‐cell level are demonstrated using a mixed‐species experiment. This platform offers a powerful way to investigate the genotype‐phenotype relationships of various mtDNA mutations in biological processes. The HetHydrogel platform is believed to have the potential to democratize droplet technologies, upgrading a whole range of plate‐based single cell assays to high throughput format.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Cited by
1 articles.
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