Larvae of Colorado potato beetle (Leptinotarsa decemlineata Say) resistant to double‐stranded RNA (dsRNA) remain susceptible to small‐molecule pesticides

Abstract

AbstractBACKGROUNDImplementation of resistance management tools is crucial for the continued efficacy of insect control technologies. An important aspect of insect resistance management (IRM) is the combined or sequential use of different modes‐of‐action to reduce selection pressure and delay evolution of resistance. This is especially important for insect pests with established ability to develop resistance to insecticides, such as the Colorado potato beetle (Leptinotarsa decemlineata, CPB). A new class of insecticides, based on double‐stranded RNA (dsRNA) activating the gene silencing RNA‐interference (RNAi) pathway, are currently under review for regulatory approval and commercial use in the USA against CPB. However, there is no information available on the potential for cross‐resistance between RNAi insecticides and other classes of insecticides used against CPB. Herein, we aim to fill this knowledge gap by capitalizing on the availability of a CPB strain highly resistant to dsRNAs and test its susceptibility to diverse small‐molecule insecticide classes compared to reference dsRNA‐susceptible CPB strains.RESULTSDifferences in activity were observed among the four insecticides tested, with abamectin demonstrating highest activity against all three strains of CPB. However, no differences were observed among the dsRNA‐resistant and susceptible CPB strains for any of the tested compounds. Overall, these results demonstrate lack of cross‐resistance to commonly used chemical insecticides in the dsRNA‐resistant strain of CPB.CONCLUSIONThese data support the use of these different insecticide classes along with RNAi‐based insecticides as part of an effective insect resistance management framework aimed at delaying resistance in CPB. © 2023 Society of Chemical Industry.