RNA Secondary Structure Analysis Using RNAstructure

Author:

Ali Sara E.1,Mittal Abhinav1,Mathews David H.1

Affiliation:

1. Department of Biochemistry & Biophysics and Center for RNA Biology University of Rochester Medical Center Rochester New York

Abstract

AbstractRNAstructure is a user‐friendly program for the prediction and analysis of RNA secondary structure. It is available as a web server, a program with a graphical user interface, or a set of command line tools. The programs are available for Microsoft Windows, macOS, or Linux. This article provides protocols for prediction of RNA secondary structure (using the web server, the graphical user interface, or the command line) and high‐affinity oligonucleotide binding sites to a structured RNA target (using the graphical user interface). © 2023 Wiley Periodicals LLC.Basic Protocol 1: Predicting RNA secondary structure using the RNAstructure web serverAlternate Protocol 1: Predicting secondary structure and base pair probabilities using the RNAstructure graphical user interfaceAlternate Protocol 2: Predicting secondary structure and base pair probabilities using the RNAstructure command line interfaceBasic Protocol 2: Predicting binding affinities of oligonucleotides complementary to an RNA target using OligoWalk

Publisher

Wiley

Subject

Medical Laboratory Technology,Health Informatics,General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

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