Dietary supplementation of black cumin (Nigella sativa) meal in the formulation of protein‐enriched cookies, further in vivo evaluation of protein quality with physicochemical and organoleptic characterization

Author:

Batool Rizwana1,Ramzan Rabia12,Raza Awais3ORCID,Aziz Mahwash1,Rohi Madiha1,Naeem Adan1,Nusrat Wajeeha1,Razi Aiza1,Saleem Bakhtawar1,Batool Wajeeha1,Bilal Ahmed4,Khadijah Babirye5ORCID

Affiliation:

1. Department of Food Science and Technology Government College Women University Faisalabad Pakistan

2. College of Food Science and Technology Huazhong Agricultural University Wuhan China

3. University Institute of Food Science and Technology, Faculty of Allied Health Sciences The University of Lahore Lahore Pakistan

4. University Institute of Diet and Nutritional Sciences, Faculty of Allied Health Sciences The University of Lahore Lahore Pakistan

5. Department of Food Science and Nutrition Islamic University in Uganda Mbale Uganda

Abstract

AbstractNigella sativa has solid historical importance as a medicinal herb and is widely used in the bakery industry. The study revealed that the defatted black cumin meal (DBCM) possesses a high nutritious composition and amino acid profile, such as Leu, Lys, Arg, Phe, and Glu. The supplementation effect of wheat flour with defatted black cumin meal (DBCM) at levels of 5%–25% was explored on the cookies' nutritional, functional, and organoleptic attributes. Moreover, the present work was conducted to assess the in vivo protein quality of defatted black cumin meal (DBCM)‐supplemented cookies using the albino rat's experimental modeling for 10 days. Rheological characterization suggested a solid elastic behavior for all cookies dough with elevation in G' (138,652–230,926 Pa), G" (34201–45,092 Pa), and G* values due to protein addition. The functional characteristics of the flour blends showed that DBCM addition significantly (p ≤ .05) improved oil (70.56%–165%) and water (84.83%–232.67%) absorption capacities, emulsion, and foaming stability. The physicochemical and organoleptic assessment of the cookies exposed that a 20% level of substitution of DBCM produced acceptable cookies. The net protein utilization (NPU), protein efficiency ratio (PER), true digestibility (TD), and biological value (BV) significantly differ from each other among all the supplementation diet groups with 0%–25% DBCM.

Publisher

Wiley

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