Circ_001422 aggravates osteosarcoma progression through targeting miR‐497‐5p/E2F3 axis

Abstract

AbstractCircular RNAs exert vital functions in the pathogenesis of osteosarcoma (OS). Circ_001422 has been confirmed to be involved in regulating OS progression, but its specific mechanism has not been clearly studied. This work aimed to analyze circ_001422's role in OS cell biological behaviors and the possible molecular mechanisms. This work carried out reverse transcription‐quantitative polymerase chain reaction for detecting circ_001422, E2F3 and miR‐497‐5p levels, whereas Cell counting kit‐8 together with Transwell assays for measuring cell growth, migration as well as invasion abilities. Relation of miR‐497‐5p with E2F3, as well as circ_001422 with miR‐497‐5p was analyzed through dual‐luciferase reporter gene assay. Protein level was identified by western blot. According to our results, circ_001422 expression within OS tissue significantly increased compared with corresponding healthy samples. Inhibition of circ_001422 significantly decreased OS cell growth, invasion and migration. From mechanism research, miR‐497‐5p was proved as circ_001422's target, and E2F3 was miR‐497‐5p's target. Besides, miR‐497‐5p downregulation or E2F3 overexpression abolished circ_001422 inhibition‐mediated inhibition on OS cell proliferation, invasion and migration. Collectively, this study has first suggested circ_001422's role in enhancing OS proliferation, migration as well as invasion via miR‐497‐5p/E2F3 axis. Our results will offer new ideas and new anti‐OS targets.