Setting Up the JBrowse 2 Genome Browser

Author:

Diesh Colin1ORCID,Buels Robert1ORCID,Stevens Garrett1ORCID,Bridge Caroline2ORCID,Cain Scott2ORCID,Stein Lincoln2ORCID,Holmes Ian1ORCID

Affiliation:

1. Department of Bioengineering University of California Berkeley California

2. Ontario Institute for Cancer Research Toronto Ontario Canada

Abstract

AbstractJBrowse 2 is a modular genome browser that can visualize many common genomic file formats. While JBrowse 2 supports a variety of different usages, it is particularly suited for deployment on websites, such as model organism databases or other web‐based genomic data resources. This protocol provides detailed instructions for setting up JBrowse 2 on an Ubuntu Linux web server, loading a reference genome from a FASTA format file, and adding a gene annotation track from a GFF3 format file. By the end of the protocol, users will have a working JBrowse 2 instance that is accessible via the web. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Setting up JBrowse 2 on your web server

Funder

National Institutes of Health

Publisher

Wiley

Reference6 articles.

1. Diesh C. Stevens G. Xie P. De Jesus Martinez T. Hershberg E. Leung A. Guo E. Dider S. Zhang J. Bridge C. Hogue G. Duncan A. Morgan M. Flores T. Bimber B. Haw R. Cain S. Buels R. Stein L. &Holmes I.(2023).Jbrowse 2. Zenodo.https://doi.org/10.5281/ZENODO.7710472

2. Tabix: fast retrieval of sequence features from generic TAB-delimited files

3. The Sequence Alignment/Map format and SAMtools

4. Integrative genomics viewer

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