A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles

Author:

He Yao1ORCID,Yang Jie2,Hu Fengying3,Liao Min2,Nie Yuru3,Zhu Xiaoxia2,Zhang Tao4,Song Keer5,Li Qinxi1,Li Xiaojie1,Mei Chenghan1,Wu Zhe3,Lu Qiang2,Zhong Zhihui1ORCID

Affiliation:

1. Laboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital Sichuan University Chengdu China

2. Department of Medical Ultrasound, West China Hospital Sichuan University Chengdu China

3. School of Life Science and Technology University of Electronic Science and Technology of China Chengdu China

4. School of Bioscience and Technology Chengdu Medical College Chengdu China

5. Franklin College of Arts and Science University of Georgia Athens Georgia USA

Abstract

AbstractBackgroundWe aimed to prepare a non‐invasive, reproducible, and controllable rat model of intracerebral hemorrhage with focused ultrasound (FUS).MethodsA rat intracerebral hemorrhage (ICH) model was established by combining FUS and microbubbles (μBs), and edaravone was used to verify whether the free radical scavenger had a protective effect on the model. The brain tissue of each group was sectioned to observe the gross histology, blood–brain barrier (BBB) permeability, cerebral infarction volume, and histopathological changes.ResultsCompared with the FUS group, the BBB permeability was significantly increased in the FUS + μBs (F&B) group (p = 0.0021). The second coronal slice in the F&B group had an obvious hemorrhage lesion, and the FUS + μBs + edaravone (F&B&E) group had smaller hemorrhage areas; however, ICH did not occur in the FUS group. The cerebral infarction volume in the F&B group was significantly larger than that in the FUS group (p = 0.0030) and F&B&E group (p = 0.0208). HE staining results showed that nerve fibrinolysis, neuronal necrosis, microglia production, and erythrocytes were found in both the F&B group and the F&B&E group, but the areas of the nerve fibrinolysis and neuronal necrosis in the F&B group were larger than the F&B&E group.ConclusionsA rat ICH model was successfully prepared using the μBs assisted FUS treatment, and edaravone had a therapeutic effect on this model. This model can be used to study the pathophysiological mechanism of ICH‐related diseases and in preclinical research on related new drugs.

Publisher

Wiley

Subject

Medical Laboratory Technology,Veterinary (miscellaneous),Molecular Biology,Biochemistry,Medicine (miscellaneous)

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