Affiliation:
1. State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences Urumqi China
2. Pharmaceutical Preparation Laboratory Xinjiang Medicine Research Institute Urumqi China
3. Xinjiang Technical Institute of Physics and Chemistry University of Chinese Academy of Sciences Beijing China
4. Engineering Research Center for Development and High Value Utilization of Genuine Medicinal Materials in North Anhui Province School of Biological and Food Engineering, Suzhou University Suzhou China
5. Department of Pharmacology and therapeutics, Faculty of Pharmacy University of Central Punjab Lahore Pakistan
Abstract
AbstractDracocephalum Moldavica L. is a traditional herb for improving pharynx and relieving cough. However, the effect on pulmonary fibrosis is not clear. In this study, we explored the impact and molecular mechanism of total flavonoid extract from Dracocephalum moldavica L. (TFDM) on bleomycin‐induced pulmonary fibrosis mouse model. Lung function testing, lung inflammation and fibrosis, and the related factors were detected by the lung function analysis system, HE and Masson staining, ELISA, respectively. The expression of proteins was studied through Western Blot, immunohistochemistry, and immunofluorescence while the expression of genes was analyzed by RT‐PCR. The results showed that TFDM significantly improved lung function in mice, reduced the content of inflammatory factors, thereby reducing the inflammation. It was found that expression of collagen type I, fibronectin, and α‐smooth muscle actin was significantly decreased by TFDM. The results further showed that TFDM interferes with hedgehog signaling pathway by decreasing the expression of Shh, Ptch1, and SMO proteins and thereby inhibiting the generation of downstream target gene Gli1 and thus improving pulmonary fibrosis. Conclusively, these findings suggest that TFDM improve pulmonary fibrosis by reducing inflammation and inhibition of the hedgehog signaling pathway.
Funder
National Natural Science Foundation of China
Cited by
4 articles.
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