Visualization of single proteins from stripped native cell membranes: A protocol for high-resolution atomic force microscopy

Author:

Marasini Carlotta1,Jacchetti Emanuela,Moretti Manola2,Canale Claudio2,Moran Oscar1,Vassalli Massimo1

Affiliation:

1. Istituto di Biofisica; Consiglio Nazionale delle Ricerche; Genova; 16149; Italy

2. Nanophysics Dipartimento; Istituto Italiano di Tecnologia; Morego; Genova; 16163; Italy

Publisher

Wiley

Subject

Medical Laboratory Technology,Instrumentation,Histology,Anatomy

Reference28 articles.

1. An adhesion-based method for plasma membrane isolation: Evaluating cholesterol extraction from cells and their membranes;Bezrukov;Anal Biochem,2009

2. Applicability of different antibodies for immunohistochemical localization of CFTR in sweat glands from healthy controls and from patients with cystic fibrosis;Claass;J Histochem Cytochem,2000

3. Membrane isolation on polylysine-coated beads: Plasma membrane from HeLa cells;Cohen;J Cell Biol,1977

4. Nanoscale structural and mechanical effects of beta-amyloid (1-42) on polymer cushioned membranes: A combined study by neutron reflectometry and AFM force spectroscopy;Dante;Biochim Biophys Acta,2011

5. The atomic force microscope detects ATP-sensitive protein clusters in the plasma membrane of transformed MDCK cells;Ehrenhofer;Cell Biol Int,1997

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