Affiliation:
1. Key Laboratory of Biorheological Science and Technology Ministry of Education College of Bioengineering Chongqing University Chongqing 400044 P. R. China
2. Department of Orthopedics The Second Affiliated Xinqiao Hospital of Army Medical University Chongqing 400030 P. R. China
Abstract
AbstractOsteonecrosis of the femoral head (ONFH) is a common but sophisticated multifactorial disease. In this study, the genomic characteristics of ONFH from the sequencing is first analyzed. Excepting ischemia and inflammation, some of differentially expressed genes (DEGs) are connected with autophagy. So, they are taken as the breakthrough points to design an auto polymerization coating of caffeic acid with grafting deferoxamine (DFO) and strontium on Ti substrate. In vitro cells and in vivo immunofluorescence and immunohistochemical results prove that the modified Ti can upregulate the expression of HIF‐1α in mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs) by receptors of TGF‐β and TNFR on cell membrane. Interestingly, in MSCs, the upregulated HIF‐1α can promote autophagy to some extent by HIF‐1α/BNIP2 pathway. Meanwhile, appropriate autophagy and HIF‐1α can regulate MSCs proliferation and osteogenic differentiation by STAT3/Notch pathway. However, in HUVECs, although autophagy increases slightly, the upregulated HIF‐1α prefers to increase its downstream target gene VEGF and accelerate angiogenesis by the pathway of HIF‐1α/VEGF. The results of in vivo implantation show that the modified Ti substrate can effectively enhance the integration effect of the implant by osteogenesis and angiogenesis. More significantly, the modified Ti can retard the further deterioration of ONFH by regulation of key genes.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Chongqing
Major Research Plan
Key Programme
National Outstanding Youth Science Fund Project of National Natural Science Foundation of China
Subject
Electrochemistry,Condensed Matter Physics,Biomaterials,Electronic, Optical and Magnetic Materials
Cited by
3 articles.
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