Affiliation:
1. Department of Oral Biology, The Goldschleger School of Dental Medicine, Faculty of Medicine Tel Aviv University Tel Aviv Israel
2. The Center for Nanoscience and Nanotechnology, Tel Aviv University Tel Aviv Israel
3. The Center for the Physics and Chemistry of Living Systems, Tel Aviv University Tel Aviv Israel
4. School of Plant Sciences and Food Security, The George S. Wise Faculty of Life Sciences Tel Aviv University Tel Aviv Israel
Abstract
AbstractRedox–enzyme‐mediated electrochemical processes such as hydrogen production, nitrogen fixation, and CO2 reduction are at the forefront of the green chemistry revolution. To scale up, the inefficient two‐dimensional (2D) immobilization of redox enzymes on working electrodes must be replaced by an efficient dense 3D system. Fabrication of 3D electrodes was demonstrated by embedding enzymes in polymer matrices. However, several requirements, such as simple immobilization, prolonged stability, and resistance to enzyme leakage, still need to be addressed. The study presented here aims to overcome these gaps by immobilizing enzymes in a supramolecular hydrogel formed by the self‐assembly of the peptide hydrogelator fluorenylmethyloxycarbonyl‐diphenylalanine. Harnessing the self‐assembly process avoids the need for tedious and potentially harmful chemistry, allowing the rapid loading of enzymes on a 3D electrode under mild conditions. Using the [FeFe] hydrogenase enzyme, high enzyme loads, prolonged resistance against electrophoresis, and highly efficient hydrogen production are demonstrated. Further, this enzyme retention is shown to arise from its interaction with the peptide nanofibrils. Finally, this method is successfully used to retain other redox enzymes, paving the way for a variety of enzyme‐mediated electrochemical applications.
Funder
Israel Science Foundation
Subject
Materials Chemistry,Energy (miscellaneous),Materials Science (miscellaneous),Renewable Energy, Sustainability and the Environment
Cited by
1 articles.
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1. Economic Optimization of the Hydrogen Demand in a Hard-to-Abate Industrial Sector;2023 Asia Meeting on Environment and Electrical Engineering (EEE-AM);2023-11-13