Scalable, two‐stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion-Reference-Cited by-同舟云学术

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Scalable, two‐stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion

Published:2020-06-10 Issue:9 Volume:117 Page:2715-2727
ISSN:0006-3592
Container-title:Biotechnology and Bioengineering
language:en
Short-container-title:Biotechnology and Bioengineering

Author:

Menacho‐Melgar Romel¹,Ye Zhixia¹,Moreb Eirik A.¹,Yang Tian¹,Efromson John P.¹,Decker John S.¹,Wang Ruixin¹,Lynch Michael D.¹^ORCID

Affiliation:

1. Department of Biomedical Engineering Duke University Durham North Carolina

Funder

Defense Advanced Research Projects Agency

Foundation for the National Institutes of Health

Office of Naval Research

North Carolina Biotechnology Center

National Science Foundation

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.27440

Reference60 articles.

1. Phosphate-Responsive Promoter of a Pichia pastoris Sodium Phosphate Symporter

2. A gene cluster encoding malonyl-CoA decarboxylase (MatA), malonyl-CoA synthetase (MatB) and a putative dicarboxylate carrier protein (MatC) in Rhizobium trifolii . Cloning, sequencing, and expression of the enzymes in Escherichia coli

3. Short, auto-inducible promoters for well-controlled protein expression in Escherichia coli

4. Directed Evolution of Methanococcus jannaschii Citramalate Synthase for Biosynthesis of 1-Propanol and 1-Butanol by Escherichia coli

5. Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection

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