Adipose-derived mesenchymal stem cells in biosutures do not improve healing of experimental colonic anastomoses-Reference-Cited by-同舟云学术

Adipose-derived mesenchymal stem cells in biosutures do not improve healing of experimental colonic anastomoses

Published:2008-08-08 Issue:9 Volume:95 Page:1180-1184
ISSN:0007-1323
Container-title:British Journal of Surgery
language:en
Short-container-title:

Author:

Pascual I¹²³,de Miguel G Fernández⁴,Gómez-Pinedo U A⁵⁶⁷,de Miguel F²,Arranz M García²,García-Olmo D¹²³

Affiliation:

1. Department of General Surgery, La Paz University Hospital, Madrid, Valencia, Spain

2. Cell Therapy Unit, La Paz University Hospital, Madrid, Valencia, Spain

3. Department of Surgery, Autonoma University of Madrid, Valencia, Spain

4. Cellerix, Madrid, Valencia, Spain

5. Centro de Investigación Príncipe Felipe, Valencia, Spain

6. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco/Consejo Nacional de Ciencia y Tecnología, Guadalajara, Mexico

7. Organismo Público Descentralizado Hospital Civil de Guadalajara, Guadalajara, Mexico

Abstract

Abstract Background The feasibility of producing ‘biosutures’ coated with adipose-derived mesenchymal stem cells (ASCs) was assessed and their effect on colonic anastomoses evaluated. Methods Sutures were cultured with ASCs isolated from BDIX rats before analysis by light and scanning electron microscopy. Anastomoses were performed in 40 BDIX rats using either biosutures or conventional sutures. Histopathological features, adhesion formation and anastomotic strength were evaluated. Results Sutures were coated with ASCs within 24 h. Anastomoses made with biosutures had a lower adhesion index only during the first week (P = 0·006 at 7 days), but not subsequently. There were no significant differences in anastomotic healing with the two types of suture. Conclusion ASC biosutures have no effect on anastomotic healing in rats.

Funder

Cellerix to the Chair of Cell Therapy and Regenerative Medicine, Autonoma University of Madrid

Publisher

Oxford University Press (OUP)

Subject

Surgery

Link

http://academic.oup.com/bjs/article-pdf/95/9/1180/36709661/bjs6242.pdf

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