Functional analysis of yeast essential genes using a promoter‐substitution cassette and the tetracycline‐regulatable dual expression system
Author:
Affiliation:
1. Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina, Universitat de Lleida, Rovira Roure 44, 25198‐Lleida, Spain
Publisher
Wiley
Subject
Genetics,Applied Microbiology and Biotechnology,Biochemistry,Bioengineering,Biotechnology
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/%28SICI%291097-0061%2819980915%2914%3A12%3C1127%3A%3AAID-YEA300%3E3.0.CO%3B2-%23
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1. Sequence analysis of a 12 801 bp fragment of the left arm of yeast chromosome XV containing a putative 6-phosphofructo-2-kinase gene, a gene for a possible glycophospholipid-anchored surface protein and six other open reading frames
2. TOR controls translation initiation and early G1 progression in yeast.
3. An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast [published erratum appears in Nucleic Acids Res 1998 Apr 1;26(7):following 1855]
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