Daboxin P, a phospholipase A2 of Indian Daboia russelii venom, modulates thrombin‐mediated platelet aggregation
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Published:2023-07-19
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Volume:
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ISSN:1095-6670
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Container-title:Journal of Biochemical and Molecular Toxicology
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language:en
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Short-container-title:J Biochem & Molecular Tox
Author:
Yasmin Rafika1,
Chanchal Shankar2,
Ashraf Mohammad Zahid2,
Doley Robin1ORCID
Affiliation:
1. Department of Molecular Biology and Biotechnology Tezpur University Tezpur Assam India
2. Department of Biotechnology Faculty of Natural Sciences, Jamia Millia Islamia New Delhi New Delhi India
Abstract
AbstractDaboxin P, reported earlier from the venom of Daboia russellii, disturbs the blood coagulation cascade by targeting factor X and factor Xa. The present study exhibits that Daboxin P also inhibits platelet aggregation induced by various agonists. The thrombin‐induced platelet aggregation was inhibited maximum whereas inhibition of collagen‐induced platelet aggregation was found to be 50% and no inhibition of adenosine diphosphate (ADP) and arachidonic acid‐induced aggregation was observed. Daboxin P dose‐dependently inhibited the thrombin‐induced platelet aggregation with Anti‐Aggregation 50 (AD50) dose of 55.166 nM and also reduced the thrombin‐mediated calcium influx. In‐silico interaction studies suggested that Daboxin P binds to thrombin and blocks its interaction with its receptor on the platelet surface. Quenching of thrombin's emission spectrum by Daboxin P and electrophoretic profiles of pull‐down assay further reveals the binding between Daboxin P and thrombin. Thus, the present study demonstrates that Daboxin P inhibits thrombin‐induced platelet aggregation by binding to thrombin.
Funder
Department of Biotechnology, Ministry of Science and Technology, India
Subject
Health, Toxicology and Mutagenesis,Toxicology,Molecular Biology,Molecular Medicine,Biochemistry,General Medicine