Improved gap repair cloning in yeast: treatment of the gapped vector withTaqDNA polymerase avoids vector self-ligation-Reference-Cited by-同舟云学术

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Improved gap repair cloning in yeast: treatment of the gapped vector withTaqDNA polymerase avoids vector self-ligation

Published:2012-09-27 Issue:10 Volume:29 Page:419-423
ISSN:0749-503X
Container-title:Yeast
language:en
Short-container-title:Yeast

Author:

Bessa Daniela,Pereira Filipa¹,Moreira Roxana²,Johansson Björn¹,Queirós Odília²

Affiliation:

1. CBMA - Center of Molecular and Environmental Biology; Department of Biology; University of Minho; Braga; 4710-057; Portugal

2. Centro de Investigação em Ciências da Saúde (CICS); Instituto Superior de Ciências da Saúde-Norte/CESPU; 4585-116; Gandra; PRD; Portugal

Publisher

Wiley

Subject

Genetics,Applied Microbiology and Biotechnology,Biochemistry,Bioengineering,Biotechnology

Reference12 articles.

1. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae;Baudin;Nucleic Acids Res,1993

2. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method;Gietz;Methods Enzymol,2002

3. DNA polymerase-catalyzed addition of nontemplated extra nucleotides to the 3′ end of a DNA fragment;Hu;DNA Cell Biol,1993

4. Drag&Drop cloning in yeast;Jansen;Gene,2005

5. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds;Mumberg;Gene,1995

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