Disclosing quantitative <scp>RT‐PCR</scp> raw data during manuscript submission: a call for action-Reference-Cited by-同舟云学术

Disclosing quantitative RT‐PCR raw data during manuscript submission: a call for action

Published:2023-03-27 Issue:5 Volume:17 Page:713-717
ISSN:1574-7891
Container-title:Molecular Oncology
language:en
Short-container-title:Molecular Oncology

Author:

Untergasser Andreas¹²,Hellemans Jan³,Pfaffl Michael W.⁴,Ruijter Jan M.⁵,van den Hoff Maurice J. B.⁵,Dragomir Mihnea P.⁶⁷⁸,Adamoski Douglas⁹,Dias Sandra Martha Gomes⁹,Reis Rui Manuel¹⁰¹¹¹²^ORCID,Ferracin Manuela¹³¹⁴^ORCID,Dias‐Neto Emmanuel¹⁵¹⁶,Marsh Ian¹⁷,Kubista Mikael¹⁸¹⁹,Fabbri Muller²⁰,Goel Ajay²¹²²^ORCID,Slabý Ondřej²³²⁴,Knutsen Erik²⁵^ORCID,Chen Baoqing²⁶,Negrini Massimo²⁷,Mimori Koshi²⁸,Pichler Martin²⁹³⁰,Papatriantafyllou Maria³¹,Anfossi Simone³²,Schmittgen Thomas D.³³,Huggett Jim³⁴³⁵,Bustin Stephen³⁶,Vandesompele Jo³³⁷³⁸,Calin George A.³²³⁹^ORCID,

Affiliation:

1. Zentrum für Molekulare Biologie der Universität Heidelberg Germany

2. Genomics Core Facility European Molecular Biology Laboratory (EMBL) Heidelberg Germany

3. CellCarta Zwijnaarde Belgium

4. Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan Technical University of Munich Freising Germany

5. Department of Medical Biology, Amsterdam UMC, location AMC University of Amsterdam The Netherlands

6. Institute of Pathology, Berlin Institute of Health Charité ‐ Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt‐Universität zu Berlin Germany

7. Berlin Institute of Health Germany

8. German Cancer Research Center (DKFZ) German Cancer Consortium (DKTK) Heidelberg Germany

9. Brazilian Biosciences National Laboratory (LNBio) Brazilian Center for Research in Energy and Materials (CNPEM) Campinas Brazil

10. Molecular Oncology Research Center Barretos Cancer Hospital Brazil

11. Life and Health Sciences Research Institute (ICVS), School of Health Sciences University of Minho Braga Portugal

12. 3B's‐PT Government Associate Laboratory Braga/Guimarães Portugal

13. Department of Medical and Surgical Sciences (DIMEC) University of Bologna Italy

14. IRCCS Azienda Ospedaliero‐Universitaria di Bologna Italy

15. Laboratory of Medical Genomics Centro Internacional de Pesquisa, AC Camargo Cancer Center São Paulo Brazil

16. Laboratory of Neurosciences (LIM27), Institute of Psychiatry, Faculdade de Medicina Universidade de São Paulo Brazil

17. New South Wales Department of Primary Industries Elizabeth Macarthur Agricultural Institute Narellan Australia

18. TATAA Biocenter AB Göteborg Sweden

19. Institute of Biotechnology CAS, v. v. i. Vestec Czech Republic

20. Center for Cancer and Immunology Research Children's National Hospital Washington DC USA

21. Department of Molecular Diagnostics and Experimental Therapeutics Beckman Research Institute of City of Hope Monrovia CA USA

22. City of Hope Comprehensive Cancer Center Duarte CA USA

23. Department of Biology, Faculty of Medicine, Central European Institute of Technology Masaryk University Brno Czech Republic

24. Department of Comprehensive Cancer Care Masaryk Memorial Cancer Institute Brno Czech Republic

25. Department of Medical Biology, Faculty of Health Sciences UiT‐The Arctic University of Norway Tromsø Norway

26. Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine Sun Yat‐sen University Cancer Center Guangzhou China

27. Laboratory for Technologies of Advanced Therapies (LTTA), Department of Morphology, Surgery and Experimental Medicine University of Ferrara Italy

28. Department of Surgery Kyushu University Beppu Hospital Japan

29. Division of Oncology, Department of Internal Medicine Medical University of Graz Austria

30. Translational Oncology University Hospital of Augsburg Stenglinstrasse Germany

31. Editorial Manager Molecular Oncology Cambridge UK

32. Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center Houston TX USA

33. Department of Pharmaceutics University of Florida College of Pharmacy Gainesville FL USA

34. National Measurement Laboratory LGC Teddington UK

35. School of Biosciences and Medicine, Faculty of Health and Medical Sciences University of Surrey Guildford UK

36. Medical Technology Research Centre Anglia Ruskin University Chelmsford Essex UK

37. Department of Biomolecular Medicine Ghent University Belgium

38. OncoRNALab Cancer Research Institute Ghent (CRIG) Belgium

39. The Center for Non‐codingRNAs University of Texas MD Anderson Cancer Center Houston TX USA

Abstract

Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT‐qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT‐qPCR raw data. The Real‐time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.

Funder

Fundação de Amparo à Pesquisa do Estado de São Paulo

National Multiple Sclerosis Society Michigan Chapter

Publisher

Wiley

Subject

Cancer Research,Genetics,Molecular Medicine,General Medicine,Oncology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/1878-0261.13418

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