An Acid‐Activatable Fluorescent Probe for Sulfur Dioxide in Traditional Chinese Medicines and Living Cells

Author:

Xi Guan123ORCID,Liu Mei14,Zhou Peitao5,Yu Congting14,Zhang Fan123,Zhang Zhenqiang14,Zhang Wenli14,Luan Tiangang123

Affiliation:

1. Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Jieyang 515200 China

2. School of Biomedical and Pharmaceutical Sciences Guangdong University of Technology 100 Waihuanxi Road Guangzhou 510006 China

3. Smart Medical Innovation Technology Center School of Biomedical and Pharmaceutical Sciences Guangdong University of Technology 100 Waihuanxi Road Guangzhou 510006 China

4. School of Chemical Engineering and Light Industry Guangdong University of Technology 100 Waihuanxi Road Guangzhou 510006 China

5. Department of Radiation Oncology Nanfang Hospital Southern Medical University Guangzhou 510515 China

Abstract

AbstractExcessive sulfur dioxide (SO₂) disturbs physiology of lysosomes causing diseases and threatening human health. A fluorescent probe has been regarded as one of the most attractive approaches, which is compatible with living cells and possesses high sensitivity. However, most of fluorescent probes’ reaction sites are activated before they reach the destination. In this work, an acid‐activatable fluorescent probe PT1 was synthesized, characterized, and used for SO2 detection. The introduction of oxazolines in PT1 enables the intelligent response of probe to release the activation stie for SO2 derivatives through Michael addition upon exposure to acid. In vitro studies showed a remarkable selectivity of PT1 to SO₂ derivatives than other biothiols with a limit of detection as low as 62 nM. By using this acidic pH‐controlled fluorescence responsiveness to SO₂, precise spatiotemporal identification of lysosomal SO2 fluctuations has been successfully performed. Furthermore, probe PT1 can be applied for monitoring SO₂ derivatives in traditional Chinese medicines.

Funder

National Natural Science Foundation of China

Publisher

Wiley

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