Fluorogenic Monitoring of α‐Amylase in Human Urine for Straightforward Diagnosis of Pancreatic Diseases

Abstract

AbstractIn this study, we developed a sensitive method for monitoring α‐amylase using a fluorogenic approach based on the host‐guest complexation between an amphiphilic pyrenyl derivative (1) and γ‐cyclodextrins (γ‐CDs). The compound 1 self‐assembles into nanofibrils in aqueous solutions. Upon the introduction of γ‐CD, compound 1 forms an inclusion complex with it. This complex then participates in the formation of a 2 : 2 complex with another complex, leading to strong excimer fluorescence. Upon interaction with α‐amylase, γ‐CD undergoes hydrolysis, leading to the regeneration of nanofibrils, which is accompanied by a decrease in excimer fluorescence and an increase in monomeric fluorescence. This ratiometric fluorescence color change enables the sensitive detection of low levels of α‐amylase in human urine, offering a practical approach for early screening of pancreatic‐related diseases.