Affiliation:
1. Key Laboratory for Organic Electronics and Information Displays Institute of Advanced Materials Nanjing University of Posts & Telecommunications Nanjing 210023 P. R. China
2. Key Laboratory of Flexible Electronics Institute of Advanced Materials Nanjing Tech University Nanjing 211816 P. R. China
3. Frontiers Science Center for Flexible Electronics Xi'an Institute of Flexible Electronics Northwestern Polytechnical University Xi'an 710072 P. R. China
4. Key Laboratory of Flexible Electronics of Zhejiang Province Ningbo Institute of Northwestern Polytechnical University Ningbo 315103 P. R. China
Abstract
AbstractDeveloping photostable two‐photon dyes with high brightness and negligible photocytotoxicity is of key importance in fluorescence imaging but remains a huge challenge. Here, a two‐photon dye with ultrahigh brightness and photostability is demonstrated for high‐performance long‐term two‐photon fluorescence imaging. By terminated donor engineering, the designed DBD shows a higher two‐photon absorption cross‐section (δ, 418 GM vs 329 GM) and photoluminescence quantum yield (ΦPL, 62.74% vs 54.63%) than its counterpart DBA. As a consequence, two‐photon fluorescence brightness (δ×ΦPL) of DBD exhibits a 10‐folded enhancement (262 GM vs 19 GM) in comparison with typical Coumarin 307 dye. More importantly, DBD displays ultrahigh photostability and negligible photobleaching under 10 min femtosecond laser irradiation, which stands in marked contrast to Coumarin 307. Furthermore, femtosecond transient absorption spectroscopy ascribes this ultrahigh photostability and negligible photobleaching to the inefficient intersystem crossing. With these merits, DBD can be used long‐term two‐photon fluorescence imaging in vitro.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Ningbo
Fundamental Research Funds for the Central Universities
Subject
General Chemistry,Biochemistry,Organic Chemistry