An Isothiazolanthrone‐Based Self‐Assembling Anticancer Color‐Changing Dye for Concurrent Imaging and Monitoring of Cell Viability

Abstract

AbstractWe report the photophysical properties, self‐assembly and biological evaluation of an isothiazolanthrone‐based dye, 7‐amino‐6H‐anthra[9,1‐cd]isothiazol‐6‐one (AAT), which reveals anticancer properties and can be potentially used as dye for monitoring cell viability. The solvent‐dependent photophysical studies suggest that the emission of AAT is sensitive to environment polarity due to which interesting changes in the colored emission may be observed owing to the charge transfer (CT) processes. AAT also self‐assembles to tree‐like branched morphologies and produce, a greenish emission inside the cells when imaged after short interval (15 mins) of incubation while a red fluorescence could be noted after 24 h. Interestingly, AAT also produce differential emission inside mouse normal cells as compared to its cancer cell lines since it possess anticancer activity. The experimental observations were also validated theoretically via computational modeling.