Fabrication of Dual‐Stimuli‐Responsive Polymer Vesicles for Regulation of Enzymolysis Efficiency in A Cascade Reaction

Author:

Qiao Juan12,Ma Qian13,Cheng Cheng14,Qi Li12ORCID

Affiliation:

1. Beijing National Laboratory of Molecular Sciences Key Laboratory of Analytical Chemistry for Living Biosystems Institute of Chemistry Chinese Academy of Sciences No. 2 Zhongguancun, Beiyijie Beijing 100190 P. R. China

2. School of Chemical Sciences University of Chinese Academy of Sciences 19 A Yuquanlu Beijing 100049 P. R. China

3. School of Pharmacy Xinxiang Medical University No. 601 Jinsui Avenue (East) Xinxiang 453003 P. R. China

4. College of Chemistry & Environmental Science Hebei University No. 180 Wusi East Road Baoding 071002 P. R. China

Abstract

AbstractEnzymatic cascade reactions in confined microenvironments play important roles in cellular chemical transformation. Controlling enzymatic efficiency and eliminating substrate interference in cascade reactions is of great significance. To this end, a vesicle composed of poly(styrene‐maleic anhydride‐N‐isopropylacrylamide)(P(S−M−NIP)) and functionalized with 1,2‐bis(10,12‐ tricosadiynoyl)‐sn‐glycero‐3‐phosphocholine (DC89PC) was designed herein. Based on the thermo‐sensitive property of P(S−M−NIP) and the photo‐responsive property of DC89PC, a serial of dual‐stimuli‐responsive nanoreactors was constructed via enzymes encapsulation to tune their enzymolysis efficiencies. A kinetics study of the glucose oxidase‐encapsulated nanoreactor indicated that its enzymolysis velocity increased 2.1‐ and 1.6‐fold under heating and the ultraviolet (UV)‐light irradiation, respectively. Consequently, an enzymatic cascade reaction in the proposed enzyme reactor encapsulated with β‐galactosidase and glucose oxidase was investigated. The results revealed a 2.9‐fold enhancement in enzymolysis efficiency by changing the ambient temperature under UV irradiation. The dual‐stimuli‐responsive polymer vesicles could also eliminate H2O2 interference during the enzymatic cascade reaction. The vesicles demonstrated potential for switch‐membrane‐permeability, while, the confined microenvironment played a key role in regulating the reactions upon the temperature change and the presence of UV light. Our synthetic multi‐organelle‐like system provides a new way to mimic the control of cascade reaction catalytic processes by programming the “open/close” sates of the nanocapsules.

Funder

National Natural Science Foundation of China

Publisher

Wiley

Subject

General Chemistry,Biochemistry,Organic Chemistry

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