Affiliation:
1. Shanghai Key Laboratory of Green Chemistry and Chemical Processes School of Chemistry and Molecular Engineering East China Normal University Dongchuan Road 500 Shanghai 200241 P.R. China
2. State Key Laboratory of Precision Spectroscopy East China Normal University Dongchuan Road 500 Shanghai 200241 P.R. China
3. State Key Laboratory of Supramolecular Structure and Materials College of Chemistry and Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education College of Life Science Jilin University Qianjin Road 2699 Changchun 130012 P.R. China
4. State Key Laboratory of Bioreactor Engineering Shanghai Key Laboratory of Chemical Biology School of Pharmacy East China University of Science and Technology Meilong Road 130 Shanghai 200237 P.R. China
Abstract
AbstractA high‐density Raman photometry based on a dual‐recognition strategy is created for accurately quantifying acetylcholinesterase (AchE) activity in 24 brain regions of free‐moving animals with network. A series of 5‐ethynyl‐1,2,3,3‐tetramethyl‐based molecules with different conjugated structures and substitute groups are designed and synthesized for specific recognition of AchE by Raman spectroscopy. After systematically evaluating the recognition ability toward AchE, 2‐(4‐((4‐(dimethylamino)benzoyl)oxy)styryl)‐5‐ethynyl‐1,3,3‐trimethyl‐3H‐indol‐1‐ium (ET‐5) is finally optimized for AchE determination, which shows the highest selectivity, the greatest sensitivity, and the fastest response time among the investigated seven molecules. More interestingly, using the developed probe for AchE with high accuracy and sensitivity, the optimized AchE regulated by nitric oxide (NO) is discovered for promoting the neurogenesis of neural stem cells (NSCs). Benefiting from the high‐density photometry, it is found that the activity and distribution of AchE varied in 24 brain regions, and the levels of AchE activity in 24 brain regions of Alzheimer's mice (AD) are lower than those of normal mice. It is the first time that a functional network of AchE in 24 brain regions is established. It is also found that the loss of AchE functional network in AD mice is restored and reconstructed by the controlled release of AchE regulated by NO.
Funder
National Natural Science Foundation of China
Subject
General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)