YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles

Author:

Cassani Marco12ORCID,Fernandes Soraia12ORCID,Oliver‐De La Cruz Jorge13ORCID,Durikova Helena1ORCID,Vrbsky Jan1,Patočka Marek45ORCID,Hegrova Veronika4,Klimovic Simon6,Pribyl Jan6ORCID,Debellis Doriana7ORCID,Skladal Petr6,Cavalieri Francesca289ORCID,Caruso Frank2ORCID,Forte Giancarlo110ORCID

Affiliation:

1. International Clinical Research Center St. Anne's University Hospital Brno 60200 Czech Republic

2. Department of Chemical Engineering The University of Melbourne Parkville Victoria 3010 Australia

3. Institute for Bioengineering of Catalonia (IBEC) The Barcelona Institute for Science and Technology (BIST) Barcelona Spain

4. NenoVision Purkynova 649/127 Brno 61200 Czech Republic

5. Faculty of Mechanical Engineering Brno University of Technology Technicka 2896/2 Brno 61669 Czech Republic

6. Department of Bioanalytical Instrumentation CEITEC Masaryk University Brno 60200 Czech Republic

7. Electron Microscopy Facility Fondazione Istituto Italiano Di Tecnologia Via Morego 30 Genoa 16163 Italy

8. School of Science RMIT University Melbourne 3000 Victoria Australia

9. Dipartimento di Scienze e Tecnologie Chimiche Università di Roma “Tor Vergata” Via Della Ricerca Scientifica Rome 00133 Italy

10. School of Cardiovascular and Metabolic Medicine & Sciences King's College London London WC2R 2LS UK

Abstract

AbstractInteractions between living cells and nanoparticles are extensively studied to enhance the delivery of therapeutics. Nanoparticles size, shape, stiffness, and surface charge are regarded as the main features able to control the fate of cell‐nanoparticle interactions. However, the clinical translation of nanotherapies has so far been limited, and there is a need to better understand the biology of cell‐nanoparticle interactions. This study investigates the role of cellular mechanosensitive components in cell‐nanoparticle interactions. It is demonstrated that the genetic and pharmacologic inhibition of yes‐associated protein (YAP), a key component of cancer cell mechanosensing apparatus and Hippo pathway effector, improves nanoparticle internalization in triple‐negative breast cancer cells regardless of nanoparticle properties or substrate characteristics. This process occurs through YAP‐dependent regulation of endocytic pathways, cell mechanics, and membrane organization. Hence, the study proposes targeting YAP may sensitize triple‐negative breast cancer cells to chemotherapy and increase the selectivity of nanotherapy.

Funder

Ministerstvo Zdravotnictví Ceské Republiky

H2020 Marie Skłodowska-Curie Actions

European Regional Development Fund

Publisher

Wiley

Subject

General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3