An RNA Motif That Enables Optozyme Control and Light‐Dependent Gene Expression in Bacteria and Mammalian Cells

Author:

Pietruschka Georg1,Ranzani Américo T.2,Weber Anna13,Patwari Tejal1,Pilsl Sebastian1,Renzl Christian13,Otte David M.1,Pyka Daniel1,Möglich Andreas2ORCID,Mayer Günter13ORCID

Affiliation:

1. Life and Medical Sciences (LIMES) University of Bonn Gerhard‐Domagk‐Str.1 53121 Bonn Germany

2. Lehrstuhl für Biochemie, Photobiochemie University of Bayreuth Universitätsstraße 30 95440 Bayreuth Germany

3. Center of Aptamer Research & Development University of Bonn Gerhard‐Domagk‐Str. 1 53121 Bonn Germany

Abstract

AbstractThe regulation of gene expression by light enables the versatile, spatiotemporal manipulation of biological function in bacterial and mammalian cells. Optoribogenetics extends this principle by molecular RNA devices acting on the RNA level whose functions are controlled by the photoinduced interaction of a light‐oxygen‐voltage photoreceptor with cognate RNA aptamers. Here light‐responsive ribozymes, denoted optozymes, which undergo light‐dependent self‐cleavage and thereby control gene expression are described. This approach transcends existing aptamer‐ribozyme chimera strategies that predominantly rely on aptamers binding to small molecules. The optozyme method thus stands to enable the graded, non‐invasive, and spatiotemporally resolved control of gene expression. Optozymes are found efficient in bacteria and mammalian cells and usher in hitherto inaccessible optoribogenetic modalities with broad applicability in synthetic and systems biology.

Funder

HORIZON EUROPE European Research Council

Publisher

Wiley

Subject

General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)

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