Affiliation:
1. Department of Mechanics and Engineering Science, and Beijing Innovation Center for Engineering Science and Advanced Technology College of Engineering Peking University Beijing 100871 P. R. China
2. Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 P. R. China
3. Department of Hepatobiliary Surgery Air Force Medical Center Beijing P. R. China 100142
4. Tianjin Key Laboratory of Epigenetics for Organ Development of Premature Infants Fifth Central Hospital of Tianjin Tianjin 300450 P. R. China
5. Department of Physiology and Pathopgysiology School of Basic Medical Sciences Tianjin Medical University Tianjin 300070 P. R. China
Abstract
AbstractCell invasion/migration through three‐dimensional (3D) tissues is not only essential for physiological/pathological processes, but a hallmark of cancer malignancy. However, how to quantify spatiotemporal dynamics of 3D cell migration/invasion is challenging. Here, this work reports a 3D cell invasion/migration assay (3D‐CIMA) based on electromechanical coupling chip systems, which can monitor spatiotemporal dynamics of 3D cell invasion/migration in a real‐time, label‐free, nondestructive, and high‐throughput way. In combination with 3D topological networks and complex impedance detection technology, this work shows that 3D‐CIMA can quantitively characterize collective invasion/migration dynamics of cancer cells in 3D extracellular matrix (ECM) with controllable biophysical/biomechanical properties. More importantly, this work further reveals that it has the capability to not only carry out quantitative evaluation of anti‐tumor drugs in 3D microenvironments that minimize the impact of cell culture dimensions, but also grade clinical cancer specimens. The proposed 3D‐CIMA offers a new quantitative methodology for investigating cell interactions with 3D extracellular microenvironments, which has potential applications in various fields like mechanobiology, drug screening, and even precision medicine.
Funder
National Key Research and Development Program of China
Natural Science Foundation of Beijing Municipality
National Natural Science Foundation of China
Subject
General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)
Cited by
1 articles.
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