Affiliation:
1. Standard Bioelectronics. Co. 511 Michuhol Tower, Gaetbeol‐ro 12 Incheon 21999 South Korea
2. Department of Nano‐Bioengineering Incheon National University Academy‐ro 119 Incheon 22012 South Korea
Abstract
AbstractDNA can be used to store digital data, and synthetic short‐sequence DNA pools are developed to store high quantities of digital data. However, synthetic DNA data cannot be actively processed in DNA pools. An active DNA data editing process is developed using splint ligation in a droplet‐controlled fluidics (DCF) system. DNA fragments of discrete sizes (100–500 bps) are synthesized for droplet assembly, and programmed sequence information exchange occurred. The encoded DNA sequences are processed in series and parallel to synthesize the determined DNA pools, enabling random access using polymerase chain reaction amplification. The sequencing results of the assembled DNA data pools can be orderly aligned for decoding and have high fidelity through address primer scanning. Furthermore, eight 90 bps DNA pools with pixel information (png: 0.27–0.28 kB), encoded by codons, are synthesized to create eight 270 bps DNA pools with an animation movie chip file (mp4: 12 kB) in the DCF system.
Funder
National Research Foundation of Korea
Subject
General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)