A Platform for Assessing Cellular Contractile Function Based on Magnetic Manipulation of Magnetoresponsive Hydrogel Films

Author:

Yadid Moran12ORCID,Hagel Mario3ORCID,Labro Megan Beldjilali3ORCID,Le Roi Baptiste3,Flaxer Carina3,Flaxer Eli4,Barnea A. Ronny3,Tejman‐Yarden Shai567,Silberman Eric2,Li Xin8,Rauti Rossana9,Leichtmann‐Bardoogo Yael3,Yuan Hongyan8,Maoz Ben M.31011ORCID

Affiliation:

1. The Azrieli Faculty of Medicine Bar Ilan University 8 Henrietta Szold St. Safed 1311502 Israel

2. The Shmunis School of Biomedicine and Cancer Research Tel Aviv University Tel Aviv 69978 Israel

3. Department of Biomedical Engineering Tel Aviv University Tel Aviv 69978 Israel

4. AFEKA – Tel‐Aviv Academic College of Engineering Tel‐Aviv 69107 Israel

5. The Edmond J. Safra International Congenital Heart Center Sheba Medical Center Ramat Gan 52621 Israel

6. The Engineering Medical Research Lab Sheba Medical Center Ramat Gan 52621 Israel

7. The Sackler School of Medicine Tel Aviv University Tel Aviv 69978 Israel

8. Shenzhen Key Laboratory of Soft Mechanics and Smart Manufacturing Department of Mechanics and Aerospace Engineering Southern University of Science and Technology Shenzhen 518055 China

9. Department of Biomolecular Sciences University of Urbino Carlo Bo Urbino 61029 Italy

10. Sagol School of Neuroscience Tel Aviv University Tel Aviv 69978 Israel

11. The Center for Nanoscience and Nanotechnology Tel Aviv University Tel Aviv 69978 Israel

Abstract

AbstractDespite significant advancements in in vitro cardiac modeling approaches, researchers still lack the capacity to obtain in vitro measurements of a key indicator of cardiac function: contractility, or stroke volume under specific loading conditions—defined as the pressures to which the heart is subjected prior to and during contraction. This work puts forward a platform that creates this capability, by providing a means of dynamically controlling loading conditions in vitro. This dynamic tissue loading platform consists of a thin magnetoresponsive hydrogel cantilever on which 2D engineered myocardial tissue is cultured. Exposing the cantilever to an external magnetic field—generated by positioning magnets at a controlled distance from the cantilever—causes the hydrogel film to stretch, creating tissue load. Next, cell contraction is induced through electrical stimulation, and the force of the contraction is recorded, by measuring the cantilever's deflection. Force–length‐based measurements of contractility are then derived, comparable to clinical measurements. In an illustrative application, the platform is used to measure contractility both in untreated myocardial tissue and in tissue exposed to an inotropic agent. Clear differences are observed between conditions, suggesting that the proposed platform has significant potential to provide clinically relevant measurements of contractility.

Funder

Israel Science Foundation

Publisher

Wiley

Subject

General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)

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