Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing

Author:

Xu Yingni1,Liu Jia1,Song Wenjing1,Wang Qianchun2,Sun Xiaomin1,Zhao Qi1,Huang Yongrui1,Li Haochen1,Peng Yuehai34,Yuan Jin5,Ji Baohua6,Ren Li17ORCID

Affiliation:

1. School of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. China

2. Wenzhou Institute University of Chinese Academy of Sciences Wenzhou 325001 P. R. China

3. National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. China

4. Guangzhou Proud Seeing Biotechnology Co., Ltd Guangzhou 510320 P. R. China

5. State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat‐sen University Guangzhou 510623 P. R. China

6. Institute of Biomechanics and Applications, Department of Engineering Mechanics Zhejiang University Hangzhou 310027 P. R. China

7. Bioland Laboratory Guangzhou Regenerative Medicine and Health Guangdong Laboratory Guangzhou 510005 P. R. China

Abstract

AbstractBlindness caused by corneal damage affects millions of people worldwide, and this number continues to rise. However, rapid epithelization and a stable epithelium process are the two biggest challenges for traditional corneal materials. These processes are related to corneal curvature, which is an important factor in determination of the corneal healing process and epithelial behavior during corneal damage. In this study, smooth 3D‐printed convex corneal implants based on gelatin methacrylate and collagen are generated. As epithelium distribution and adhesion vary in different regions of the natural cornea, this work separates the surfaces into four regions and studies how cells sense topological cues on curvature. It is found that rabbit corneal epithelial cells (RCECs) seeded on steeper slope gradient surfaces on convex structures result in more aligned cell organization and tighter cell‐substrate adhesion, which can also be verified through finite element simulation and signaling pathway analysis. In vivo transplantation of convex implants result in a better fit with adjacent tissue and stronger cell adhesion than flat implants, thereby accelerating corneal epithelialization and promoting collagen fibers and neural regeneration within 180 days. Taken together, printed convex corneal implants that facilitate corneal regeneration may offer a translational strategy for the treatment of corneal damage.

Funder

National Natural Science Foundation of China

Fundamental Research Funds for the Central Universities

Publisher

Wiley

Subject

General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)

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