Affiliation:
1. Key Laboratory of Organosilicon Chemistry and Material Technology Ministry of Education College of Material Chemistry and Chemical Engineering Hangzhou Normal University Hangzhou 311121 China
2. Sino‐German Joint Research Lab for Space Biomaterials and Translational Technology School of Life Sciences Northwestern Polytechnical University Xi'an 710072 China
Abstract
AbstractPlant chloroplasts have a highly compartmentalized interior, essential for executing photocatalytic functions. However, the construction of a photocatalytic reaction compartment similar to chloroplasts in inorganic–biological hybrid systems (IBS) has not been reported. Drawing inspiration from the compartmentalized chloroplast and the phenomenon of liquid–liquid phase separation, herein, a new strategy is first developed for constructing a photocatalytic subcellular hybrid system through liquid–liquid phase separation technology in living cells. Photosensitizers and in vivo expressed hydrogenases are designed to coassemble within the cell to create subcellular compartments for synergetic photocatalysis. This compartmentalization facilitates efficient electron transfer and light energy utilization, resulting in highly effective H2 production. The subcellular compartments hybrid system (HM/IBSCS) exhibits a nearly 87‐fold increase in H2 production compared to the bare bacteria/hybrid system. Furthermore, the intracellular compartments of the photocatalytic reactor enhance the system's stability obviously, with the bacteria maintaining approximately 81% of their H2 production activity even after undergoing five cycles of photocatalytic hydrogen production. The research brings forward visionary prospects for the field of semi‐artificial photosynthesis, offering new possibilities for advancements in areas such as renewable energy, biomanufacturing, and genetic engineering.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Aeronautical Science Foundation of China
Fundamental Research Funds for the Central Universities
Cited by
1 articles.
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