Affiliation:
1. State Key Laboratory for Advanced Metals and Materials School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 P. R. China
2. Beijing National Laboratory for Molecular Sciences National Biomedical Imaging Center College of Chemistry and Molecular Engineering Peking University 292 Chengfu Road, Haidian District Beijing 100871 P. R. China
3. Photosynthesis Research Center Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 P. R. China
4. Center of Single‐Molecule Sciences Institute of Modern Optics Frontiers Science Center for New Organic Matter College of Electronic Information and Optical Engineering Nankai University 38 Tongyan Road, Jinnan District Tianjin 300350 P. R. China
Abstract
AbstractPhotosynthesis is a very important process for the current biosphere which can maintain such a subtle and stable circulatory ecosystem on earth through the transformation of energy and substance. Even though been widely studied in various aspects, the physiological activities, such as intrinsic structural vibration and self‐regulation process to stress of photosynthetic proteins, are still not in‐depth resolved in real‐time. Herein, utilizing silicon nanowire biosensors with ultrasensitive temporal and spatial resolution, real‐time responses of a single photosystem I‐light harvesting complex I (PSI‐LHCI) supercomplex of Pisum sativum to various conditions, including gradient variations in temperature, illumination, and electric field, are recorded. Under different temperatures, there is a bi‐state switch process associated with the intrinsic thermal vibration behavior. When the variations of illumination and the bias voltage are applied, two additional shoulder states, probably derived from the self‐conformational adjustment, are observed. Based on real‐time monitoring of the dynamic processes of the PSI‐LHCI supercomplex under various conditions, it is successively testified to promising nanotechnology for protein profiling and biological functional integration in photosynthesis studies.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Beijing Municipality
Subject
General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)