Fluoropolymer Coated DNA Nanoclews for Volumetric Visualization of Oligonucleotides Delivery and Near Infrared Light Activated Anti‐Angiogenic Oncotherapy
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Published:2023-09-28
Issue:32
Volume:10
Page:
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ISSN:2198-3844
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Container-title:Advanced Science
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language:en
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Short-container-title:Advanced Science
Author:
Zhang Peng1,
Guo Ranran2,
Zhang Haiting1,
Yang Wuli3ORCID,
Tian Ye1
Affiliation:
1. Biomaterials Research Center School of Biomedical Engineering Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University Guangzhou 510515 China
2. School of Biomedical Engineering Guangzhou Medical University Guangzhou 510182 China
3. State Key Laboratory of Molecular Engineering of Polymers & Department of Macromolecular Science Fudan University Shanghai 200438 China
Abstract
AbstractThe potential of microRNA regulation in oncotherapy is limited by the lack of delivery vehicles. Herein, it is shown that fluoropolymer coated DNA nanoclews (FNCs) provide outstanding ability to deliver oligonucleotide through circulation and realize near infrared (NIR) light activated angiogenesis suppression to abrogate tumors. Oligonucleotides are loaded in DNA nanoclews through sequence specific bindings and then a fluorinated zwitterionic polymer is coated onto the surface of nanoclews. Further incorporating quantum dots in the polymer coating endows the vectors with NIR‐IIb (1500–1700 nm) fluorescence and NIR light triggered release ability. The FNC vector can deliver oligonucleotides to cancer cells systemically and realize on‐demand cytosolic release of the cargo with high transfection efficiency. Taking advantage of the NIR‐IIb emission, the whole delivery process of FNCs is visualized volumetrically in vivo with a NIR light sheet microscope. Loaded by FNCs, an oligonucleotide can effectively silence the target miRNA when activated with NIR light, and inhibit angiogenesis inside tumor, leading to complete ablation of cancer. These findings suggest FNCs can be used as an efficient oligonucleotide delivery platform to modulate the expression of endogenous microRNA in gene therapy of cancer.
Funder
Postdoctoral Research Foundation of China
Program of Shanghai Academic Research Leader
Basic and Applied Basic Research Foundation of Guangdong Province
National Natural Science Foundation of China
Subject
General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)