Affiliation:
1. State Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. China
2. Emergency Department of West China Hospital Sichuan University Chengdu 610041 P.R. China
3. The Institute of Blood Transfusion Chinese Academy of Medical Sciences / Peking Union Medical College Chengdu 610052 P.R. China
Abstract
AbstractAlbeit the majority of eukaryotic genomes can be pervasively transcribed to a diverse population of lncRNAs and various subtypes of lncRNA are discovered. However, the genome‐wide study of miRNA‐derived lncRNAs is still lacking. Here, it is reported that over 800 miRNA gene‐originated lncRNAs (molncRNAs) are generated from miRNA loci. One of them, molnc‐301b from miR‐301b and miR‐130b, functions as an “RNA decoy” to facilitate dissociation of the chromatin remodeling protein SMARCA5 from chromatin and thereby sequester transcription and mRNA translation. Specifically, molnc‐301b attenuates erythropoiesis by mitigating the transcription of erythropoietic and translation‐associated genes, such as GATA1 and FOS. In addition, a useful and powerful CRISPR screen platform to characterize the biological functions of molncRNAs at large‐scale and single‐cell levels is established and 29 functional molncRNAs in hematopoietic cells are identified. Collectively, the focus is on miRNA‐derived lncRNAs, deciphering their landscape during normal hematopoiesis, and comprehensively evaluating their potential roles.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Subject
General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)