Ultra‐Rapid and Specific Gelation of Collagen Molecules for Transparent and Tough Gels by Transition Metal Complexation

Author:

Suezawa Tomoyuki1,Sasaki Naoko2,Yukawa Yuichi1,Assan Nazgul1,Uetake Yuta13,Onuma Kunishige4,Kamada Rino1,Tomioka Daisuke1,Sakurai Hidehiro13,Katayama Ryohei5,Inoue Masahiro4,Matsusaki Michiya12ORCID

Affiliation:

1. Division of Applied Chemistry, Graduate School of Engineering Osaka University 2‐1 Yamadaoka Suita Osaka 565–0871 Japan

2. Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering Osaka University 2‐1 Yamadaoka Suita Osaka 565–0871 Japan

3. Innovative Catalysis Science Division, Institute for Open and Transdisciplinary Research Initiatives (ICS‐OTRI) Osaka University 2‐1 Yamadaoka Suita Osaka 565–0871 Japan

4. Department of Clinical Bio‐resource Research and Development Kyoto University Graduate School of Medicine Kyoto 606–8304 Japan

5. Division of Experimental Chemotherapy, Cancer Chemotherapy Center Japanese Foundation for Cancer Research Tokyo 135‐8550 Japan

Abstract

AbstractCollagen is the most abundant protein in the human body and one of the main components of stromal tissues in tumors which have a high elastic modulus of over 50 kPa. Although collagen has been widely used as a cell culture scaffold for cancer cells, there have been limitations when attempting to fabricate a tough collagen gel with cells like a cancer stroma. Here, rapid gelation of a collagen solution within a few minutes by transition metal complexation is demonstrated. Type I collagen solution at neutral pH shows rapid gelation with a transparency of 81% and a high modulus of 1,781 kPa by mixing with K2PtCl4 solution within 3 min. Other transition metal ions also show the same rapid gelation, but not basic metal ions. Interestingly, although type I to IV collagen molecules show rapid gelation, other extracellular matrices  do not exhibit this phenomenon. Live imaging of colon cancer organoids in 3D culture indicates a collective migration property with modulating high elastic modulus, suggesting activation for metastasis progress. This technology will be useful as a new class of 3D culture for cells and organoids due to its facility for deep‐live observation and mechanical stiffness adjustment.

Funder

Japan Agency for Medical Research and Development

New Energy and Industrial Technology Development Organization

Publisher

Wiley

Subject

General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)

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