Affiliation:
1. Department of Biochemistry & Molecular Biology, Forensic Science Program The Pennsylvania State University University Park Pennsylvania
2. Department of Justice Jan Bashinski DNA Laboratory Richmond California
3. Mitotyping Technologies State College Pennsylvania
Abstract
AbstractThis article highlights methods used to perform DNA extraction, mitochondrial DNA quantification, multiplex PCR amplification, amplicon‐based massively parallel sequencing, and data analysis of the mitochondrial genome (mitogenome) from human hair shafts. The focus is on applications to forensic casework, but this set of protocols can be used for any purpose involving small cuttings (as small as 1 to 5 mm) of human hair shafts up to 40 years from the time of collection. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Extraction of mitochondrial DNA from human hair shaftsBasic Protocol 2: Quantification of mitochondrial DNA (copies/μl)Basic Protocol 3: Multiplex amplification of the mitogenomeBasic Protocol 4: Library preparation and sequencing of mitogenome ampliconsBasic Protocol 5: Data analysis of mitogenome haplotypes
Subject
Medical Laboratory Technology,Health Informatics,General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience