Affiliation:
1. Department of Surgery, Weill Cornell Medicine, New York, New York, USA
Abstract
Abstract
Induced pluripotent stem cells (iPSCs) derived by in vitro reprogramming of somatic cells retain the capacity to self-renew and to differentiate into many cell types. Pluripotency encompasses multiple states, with naïve iPSCs considered as ground state, possessing high levels of self-renewal capacity and maximum potential without lineage restriction. We showed previously that activation-induced cytidine deaminase (AICDA) facilitates stabilization of pluripotency during reprogramming. Here, we report that Acida−/− iPSCs, even when successfully reprogrammed, fail to achieve the naïve pluripotent state and remain primed for differentiation because of a failure to suppress fibroblast growth factor (FGF)/extracellular signal-regulated kinases (ERK) signaling. Although the mutant cells display marked genomic hypermethylation, suppression of FGF/ERK signaling by AICDA is independent of deaminase activity. Thus, our study identifies AICDA as a novel regulator of naïve pluripotency through its activity on FGF/ERK signaling. Stem Cells 2019;37:1003–1017
Significance Statement Growth factor signaling requirements that modulate pluripotent state are well studied. However, the epigenetic basis of how the dynamic state of pluripotent cells is regulated and stabilized is largely a black box. The current study is important because the results show that AICDA is essential for reprogramming to ground state. A better understanding for how to stabilize ground state pluripotent cells is of fundamental importance for the use of pluripotent cell sources in disease modeling and potential cellular therapies.
Funder
New York State Department of Health
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Developmental Biology,Molecular Medicine
Cited by
5 articles.
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