Affiliation:
1. Nanotechnology and Integrated Bioengineering Centre (NIBEC) School of Engineering Ulster University York Street Belfast, Northern Ireland, BT15 1AP UK
2. Healthcare Technology Hub School of Engineering Ulster University York Street Belfast, Northern Ireland, BT15 1AP UK
3. Dipartimento Interateno di Fisica “M. Merlin” Università degli studi di Bari Aldo Moro via Orabona 4 70126 Bari Italy
4. Institute for Photonics and Nanotechnologies (IFN) CNR via Orabona 4 70126 Bari Italy
Abstract
Understanding changes in the mechanical features of a single protein from a mutated virus while establishing its relation to the point mutations is critical in developing new inhibitory routes to tackle the uncontrollable spread of the virus. Addressing this, herein, the chemomechanical features of a single spike protein are quantified from alpha, beta, and gamma variants of SARS‐CoV‐2. Integrated amplitude‐modulation atomic force microscopy is used with dynamic force–distance curve (FDC) spectroscopy, in combination with theoretical models, to quantify Young's modulus, stiffness, adhesion forces, van der Waals forces, and the dissipative energy of single spike proteins. These obtained nanomechanical properties can be correlated with mutations in the individual proteins. Therefore, this work opens new possibilities to understand how the mechanical properties of a single spike protein relate to the viral functions. Additionally, single‐protein nanomechanical experiments enable a variety of applications that, collectively, may build up a new portfolio of understanding protein biochemistry during the evolution of viruses.
Subject
General Earth and Planetary Sciences,General Environmental Science