Iontophoresis‐Driven Microneedle Arrays Delivering Transgenic Outer Membrane Vesicles in Program that Stimulates Transcutaneous Vaccination for Cancer Immunotherapy

Author:

Wang Maoze1,Yan Ge1,Xiao Qiyao1,Zhou Nan1,Chen Hao-Ran1,Xia Wei2,Peng Lihua134ORCID

Affiliation:

1. College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 P. R. China

2. Division of Applied Materials Science Department of Materials Science and Engineering Uppsala University Uppsala 751 05 Sweden

3. Jinhua Institute of Zhejiang University Jinhua 321299 Zhejiang P. R. China

4. State Key Laboratory of Quality Research in Chinese Medicine Macau University of Science and Technology Macau 999078 P. R. China

Abstract

Transdermal delivery of antigen and chemokine proteins that activates the maturation of skin dendritic cells (DCs) and direct the migration of activated DCs to lymph and spleen is an important alternative to conventional vaccines. However, stratum corneum forms a barrier to skin penetration. The poor cellular uptake of free antigens and chemokines also limits transcutaneous immunization efficacy. In this work, a pair of iontophoresis‐driven microneedle patches is constructed, of which, two kinds of outer membrane vesicles (OMVs) derived from Escherichia coli transformed by plasmid encoding gp100 (IPMN‐G) and chemokine ligand 21 (IPMN‐C) are incorporated within microneedles, respectively. The topical application of IPMN‐G and IPMN‐C shows the effectiveness of transdermally delivering gp100 and CCL21 secreting vesicles to skin DCs. With iontophoresis as a driving generator, the release and uptake of transgenic OMVs in target cells are significantly enhanced, with transcutaneous immunization initiated. The in vivo applications of IPMN‐G and IPMN‐C with a 12 h interval retard the progression and prevent the occurrence of tumor spheroids. IPMN‐GC is shown as a promising triplatform in engineering transgenic OMV‐incorporated microneedles, driven by iontophoresis into a transcutaneous vaccine, providing a noninvasive system for the transdermal delivery of antigen and chemokine proteins for transcutaneous vaccination‐meditated immunotherapy.

Publisher

Wiley

Subject

General Earth and Planetary Sciences,General Environmental Science

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Tailoring biomaterials for vaccine delivery;Journal of Nanobiotechnology;2024-08-12

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