Biocorona on Iron Oxide Nanoparticles in a Complex Biotechnological Environment: Analysis of Proteins, Lipids, and Carbohydrates

Author:

Abarca-Cabrera Lucía1ORCID,Milinovic Olga1,Heitler Viktoria1,Rühmann Broder2,Kudermann Jürgen3,Kube Massimo45ORCID,Dietz Hendrik45ORCID,Sieber Volker2ORCID,Berensmeier Sonja1ORCID,Fraga-García Paula1ORCID

Affiliation:

1. School of Engineering and Design Department of Energy and Process Engineering Chair of Bioseparation Engineering Technical University of Munich (TUM) Boltzmannstraße 15 85748 Garching Germany

2. Chemistry of Biogenic Resources Technical University of Munich (TUM) Campus Straubing Schulgasse 16 94315 Straubing Germany

3. Catalysis Research Center (CRC) Technical University of Munich (TUM) Ernst-Otto-Fischer-Straße 1 85748 Garching Germany

4. Department of Biosciences School of Natural Sciences Technical University of Munich (TUM) Am Coulombwall 4a 85748 Garching Germany

5. Munich Institute of Biomedical Engineering Technical University of Munich (TUM) Boltzmannstraße 11 85748 Garching Germany

Abstract

Upon their introduction into a biological environment, nanoparticles are spontaneously covered by a variety of biomolecules, forming a (multi)layer called the “biocorona”. However, the interaction of small and large molecules with nanosized materials is not fully understood and in complex aqueous systems, even less, limiting their exploitation. The objective is to gain insights into the mass partitioning between the solid and the liquid phases for the most abundant groups of biological molecules in a biotechnological milieu. Herein, the biocorona composition is analyzed after the exposure of bare iron oxide nanoparticles to Microchloropsis salina lysates to evaluate the influence of the environment's pH, temperature, and ionic strength on the adsorption of proteins, lipids, and carbohydrates. Maximum adsorption capacities reach at pH 4.0 and yield 0.47, 0.08, and 0.11 g g−1 for proteins, fatty acids, and carbohydrates, respectively. The increase in ionic strength and temperature of the environment promotes protein adsorption, the decrease in temperature raises fatty acid adsorption, and acidic pHs foster the adsorption of the three types of biomolecules. Abundance of the biomolecules plays a key role in the biocorona content. This approach should lead to further studies on complex systems to modulate the adsorption at the bio–nano interface.

Publisher

Wiley

Subject

General Earth and Planetary Sciences,General Environmental Science

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