Identifying low-level sequence variants via next generation sequencing to aid stable CHO cell line screening

Author:

Zhang Sheng1,Bartkowiak Lisa1,Nabiswa Bernard1,Mishra Pratibha1,Fann John1,Ouellette David2,Correia Ivan2,Regier Dean3,Liu Junjian3

Affiliation:

1. Process Sciences Cell Culture, Abbvie Bioresearch Center; 100 Research Drive Worcester MA 01605

2. Process Sciences Analytics, Abbvie Bioresearch Center; 100 Research Drive Worcester MA 01605

3. Protein Science, Abbvie Bioresearch Center; 100 Research Drive Worcester MA 01605

Funder

Abbott

AbbVie

Publisher

Wiley

Subject

Biotechnology

Reference44 articles.

1. Production of recombinant protein therapeutics in cultivated mammalian cells;Wurm;Nat Biotechnol.,2004

2. Cell culture processes for monoclonal antibody production;Li;MAbs,2010

3. Translational effects;Rosenberger;Biologicals.,1993

4. Assessing genetic heterogeneity in production cell lines: detection by peptide mapping of a low level Tyr to Gln sequence variant in a recombinant antibody;Harris;Biotechnology (N Y),1993

5. Detection and identification of a serine to arginine sequence variant in a therapeutic monoclonal antibody;Ren;J Chromatogr B,2011

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