Engineering an <i>O</i>‐methyltransferase for the Regioselective Biosynthesis of Hesperetin Dihydrochalcone-Reference-Cited by-同舟云学术

Engineering an O‐methyltransferase for the Regioselective Biosynthesis of Hesperetin Dihydrochalcone

Published:2023-09-07 Issue:22 Volume:15 Page:
ISSN:1867-3880
Container-title:ChemCatChem
language:en
Short-container-title:ChemCatChem

Author:

Kunzendorf Andreas¹^ORCID,Zirpel Bastian²,Milke Lars²,Ley Jakob P.²^ORCID,Bornscheuer Uwe T.¹^ORCID

Affiliation:

1. Department of Biotechnology & Enzyme Catalysis Institute of Biochemistry University of Greifswald Felix-Hausdorff-Str. 4 17487 Greifswald Germany

2. Symrise AG Mühlenfeldstraße 1 37603 Holzminden Germany

Abstract

AbstractDirected evolution of the O‐methyltransferase ZgOMT from Zooshikella ganghwensis focusing on active site residues resulted in highly regioselective biocatalysts (regioisomeric ratios up to 99 : 1) for the preparation of the taste active hesperetin dihydrochalcone and related compounds. These newly constructed enzyme variants provide an attractive synthesis route for para‐methylation of catechol scaffolds, which is challenging to perform with high regioselectivity utilizing wild‐type O‐methyltransferases.

Publisher

Wiley

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Catalysis

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