Biochemical and Structural Characterization of a Uronic Acid Oxidase from Citrus sinensis

Author:

Boverio Alessandro12ORCID,van Beek Hugo L.3,Savino Simone3ORCID,Ranoux Adeline4,Huijgen Wouter J. J.4ORCID,Raaijmakers Harry W. C.4,Fraaije Marco W.1ORCID,Lončar Nikola3ORCID

Affiliation:

1. Molecular Enzymology group University of Groningen Nijenborgh 4 9747AG Groningen The Netherlands

2. Department of Biology and Biotechnology University of Pavia via Ferrata 9 27100 Pavia Italy

3. GECCO Biotech 9747 AG Groningen The Netherlands

4. Cosun RD&I, Cosun Innovation Center Kreekweg 1 4671 VA Dinteloord The Netherlands

Abstract

AbstractAldaric acids are attractive diacids that can be prepared by selective oxidation of carbohydrates. For this, effective biocatalysts are in demand. This work reports on the discovery, biochemical and structural characterization of a VAO‐type flavin‐containing carbohydrate oxidase from Citrus sinensis: URAOCs3. URAOCs3 could be overexpressed using prokaryotic and eukaryotic expression systems. Extensive biochemical characterization revealed that the enzyme displays a high thermostability and an exquisite selectivity for uronic acids, galacturonic acid and glucuronic acid. The enzyme was further investigated by determining the crystal structure. The selective oxidation of D‐galacturonic acid in a complex mixture was demonstrated, showing how URAOCs3 was found to be highly effective in selectively producing galactaric acid while leaving other carbohydrates untouched. In addition to the specific discovery of URAOCs3, these findings suggest that plant proteomes can be an interesting source for new biocatalysts.

Publisher

Wiley

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Catalysis

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