Affiliation:
1. Tianjin University of Science & Technology Tianjin 300457 P. R. China
2. Key Laboratory of Engineering Biology for Low-carbon Manufacturing Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin 300308 P. R. China
3. National Center of Technology Innovation for Synthetic Biology Tianjin 300308 P. R. China
Abstract
AbstractSurface display technology has emerged as a powerful tool for enhancing the stability, accessibility, and reusability of enzymes. Fatty acid photodecarboxylase from Chlorella variabilis NC64A (CvFAP) is capable of catalyzing decarboxylation reactions from free fatty acids to alkanes under blue light, however, its stability and reusability in catalytic cycles remain to be improved. Herein, we successfully displayed CvFAP on the surface of Escherichia coli cells utilizing full‐length ice nucleation protein as the anchor protein. By comparing the performance of the surface displayed CvFAP with whole‐cells harboring CvFAP, it was found that surface displaying of CvFAP significantly reduces the reaction time and enhances substrate accessibility, resulting in a turnover number of 9972. Also, compared to soluble enzymes, the surface displayed CvFAP exhibits enhanced stability and reusability in the decarboxylation reaction. Furthermore, the broad substrate scope of surface displayed CvFAP suggests its potential for large‐scale hydrocarbon and fatty chemicals production. Overall, our results highlight the value of surface display approach in broadening the application of photodecarboxylase.
Funder
National Key Research and Development Program of China
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Catalysis