Affiliation:
1. Department of Occupational and Environmental Health, West China School of Public Health and West China Fourth Hospital Sichuan University Chengdu 610041 China
2. Healthy Food Evaluation Research Center, West China School of Public Health and West China Fourth Hospital Sichuan University Chengdu 610041 China
Abstract
AbstractBenzo[b]fluoranthene (BbF) is a common constituent of polycyclic aromatic hydrocarbons (PAHs). While numerous studies revealed adverse effects of PAHs on human health, the health effects of individual PAHs differ, and few investigations were performed on BbF. Therefore, the present study established cytotoxicity models of human lung epithelial cells (BEAS‐2B cells) and bronchial epithelial cells (16HBE cells) exposed to BbF (10, 20, and 40 μM) for 24 h to reveal the mechanisms. Results from cytotoxicity and proliferation studies demonstrated that BbF inhibited cell growth in a dose‐dependent manner. Flow cytometric analysis showed that BbF induced the appearance of a sub G1 peak, S‐phased arrest, and apoptosis in both cells. Mechanistic investigations illustrated that BbF promoted reactive oxygen species (ROS) production, altered the expression of oxidative stress indicators, and decreased mitochondrial membrane potential. BbF also interfered with the expression of regulators associated with mitochondria disruption pathway. Taken together, these results strongly suggested that BbF inhibited cell growth and induced apoptosis in human airway epithelial cells via ROS‐mediated mitochondria disruption.
Funder
Department of Science and Technology of Sichuan Province
National Natural Science Foundation of China
Cited by
3 articles.
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