A Two‐Stage Flow Cytometry Strategy to Distinguish Single Cells from Doublets in Heterogeneous Cell Mixtures and Improve Cell Cluster Identification: Application to Human Monocyte Subpopulations
Author:
Affiliation:
1. INSERM U976 Institut de Recherche Saint Louis Paris France
2. Université de Paris Paris France
3. Current address: Department of Endocrinology, Diabetology, Nutrition, Hôpital Bichat Assistance Publique – Hôpitaux de Paris Paris France
Publisher
Wiley
Subject
Medical Laboratory Technology,Health Informatics,General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Neuroscience
Link
https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpz1.229
Reference28 articles.
1. A Public BCR Present in a Unique Dual-Receptor-Expressing Lymphocyte from Type 1 Diabetes Patients Encodes a Potent T Cell Autoantigen
2. The Challenge of Distinguishing Cell–Cell Complexes from Singlet Cells in Non‐Imaging Flow Cytometry and Single‐Cell Sorting
3. Circulating T cell-monocyte complexes are markers of immune perturbations
4. The Nightmare of a Single Cell: Being a Doublet
5. Human CD14dim Monocytes Patrol and Sense Nucleic Acids and Viruses via TLR7 and TLR8 Receptors
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